LAPP对中国对虾(Penaeus chinensis)生长、缺氧耐受力及免疫抵抗力的影响   总被引：11，自引：0，他引：11  

王伟庆  李爱杰 《海洋湖沼通报》1996,(1)

实验研究维生素C多聚磷酸酯（LAPP）添加在对虾饲料中，对中国对虾的生长、缺氧耐受力及免疫抵抗力的影响；实验进一步证明了中国对虾饲料中LAPP的最适添加量为400mg／100g饲料;本文还提出饲料中的LAPP能够提高中国对虾的缺氧耐受力，在溶氧超过3．1mg／kg海水的情况下，效果显著，在降低到2．3mg／kg海水时，作用减弱；用副溶血弧菌（Vibroparahaemolyticus）注射入虾体内，12小时内记录发现，饲料中不含LAPP的一组中国对虾，死亡率高于其它组，并且，LAPP含量最高的一组，死亡率明显低，死亡时间也出现较晚，这证明LAPP能够提高中国对虾的免疫抵抗力；本实验用注射金黄色葡萄球菌后细胞的吞噬百分率作指标，研究对虾细胞的吞噬作用，发现饲料中不含LAPP的对虾的吞噬百分率较低，随着饲料中LAPP浓度的提高，对虾血细胞的吞噬百分率逐渐升高，说明中国对虾细胞的吞噬作用逐步增强。  相似文献   

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

ASM1中污水水质参数与化学计量系数的估测     

王波 《海岸工程》2002,21(4):6-12

简要介绍了活性污泥1号模型中14个污水水质参数与5个化学计量系数的估测方法和典型取值。  相似文献   

抑制栉孔扇贝第一极体对受精卵染色体行为及胚胎倍性组成的影响   总被引：4，自引：0，他引：4  

李永仁  阙华勇  张国范 《海洋科学》2005,29(8):66-70

采用细胞松弛素B（CB）处理，栉孔扇贝（Chlamys ferreri）抑制其受精卵的第一极体（PB1），研究抑制PB1对受精卵减数分裂过程及胚胎倍性组成的影响。结果发现，抑制第一极体显著改变了受精卵的染色体行为，在第二次减数分裂过程中共发现4种典型染色体分离类型，分别是三极分离（41．7％）、二极分离（11．7％）、双二极分离（24．9％）和非同步分离（2．8％），其余的受精卵（19．0％）染色体分离行为紊乱。对4-8细胞期胚胎的倍性组成进行分析，发现处理组中含有二倍体（10．9％）、三倍体（12．5％）、四倍体（19.5％）、五倍体（12．6％）以及非整倍体（46．6％）胚胎。研究结果表明，二极分离和双二极分离分别是形成三倍体和四倍体的主要机制，而其他的染色体分离行为将主要形成非整倍体。  相似文献   

溴素的有机化学──I．2，4，6－三溴苯甲酸溴代和非溴代芳酯的合成     

蔡月琴  钱佐国  孙明昆 《中国海洋大学学报(自然科学版)》1994,(3)

用ｍ－氨基苯甲酸作原料，经溴代、重氮化、还原脱氨、酰氯化和酯化反应等步骤，合成了六种新型的三溴苯甲酸的溴代和非溴代芳酯，即；双（２，４，６－三溴苯甲酸）－２＇，３＇，５＇，６＇－四溴－１＇，４＇－苯二酯，２，４，６－三溴苯甲酸－２＇，３＇，４＇，５＇，６＇－五溴苯酯，２，４，６－三溴苯甲酸－２＇，４＇，６＇－三溴苯酯，２，４，６－三溴苯甲酸－４＇－溴苯酯，２，４，６－三溴苯甲酸－４＇－甲基苯酯和２，４，６－三溴苯甲酸苯酯．通过对产物中碳、氢、溴元素的定量分析以及红外吸收光谱和氢核磁共振谱的研究，验证了合成产物的结构。  相似文献   

Biochemical Mechanism of the Eutrophication and Its Prevention—the Deep Treatment of Waste Water and Its Denitrification and Dephosphorization     

李红山  黎松强 《海洋通报(英文版)》2003,5(1):32-39

Biochemical mechanism of forming the red tide is discussed in this paper.The existence of a large number of nitrates and phosphates in the eutrophic water is the prerequisite of explosive increase of algae and the forming of red tide.Reduction of eutrophication is an important approach to preventing the red tide.The method of deep treatment of the waste water and its denitrification and dephosphorization are introduced,and a new opinion on the red tide formation and fundamental prevention is put forward.  相似文献   

藓羽藻(Bryopsis hypnoides)核酮糖-1，5-二磷酸羧化酶／加氧酶的分离与活性测定     

田超  张炎  王广策  曾呈奎 《海洋与湖沼》2004,35(4):371-375

采用硫酸铵分部沉淀与凝胶过滤的方法，进行藓羽藻Rubisco的分离研究。结果表明，分离的藓羽藻Rubisco经SDS-聚丙烯酰胺凝胶电泳检测呈两条清晰条带，分别为Rubisco大亚基与小亚基；与菠菜相比，藓羽藻Rubisco大亚基分子量与菠菜基本相同，而小亚基较之稍大一些。藓羽藻Rubisco活力测定结果表明，Rubisco分离过程中用硫酸铵分部沉淀后活力降低许多，分离后活力有所上升，但仍比粗提液活力弱；在Rubisco活力测定过程中，藓羽藻Rubisco的活化温度与其它物种Rubisco活化的温度不同，在低温下活化效果较好。这些结果说明Rubisco的酶活力受硫酸铵的影响而且藓羽藻Rubisco相对陆地高等植物结构不稳定。  相似文献   

GIS方法在南沙群岛海区海底灾害地质研究中的应用   总被引：1，自引：0，他引：1  

唐诚  詹文欢  张毅祥 《热带海洋学报》2002,21(2):104-111

采用GIS方法，对南沙群岛海区海底灾害地质进行空间叠加分析。利用模糊数学方法，对不同灾害地质因素赋予不同的模糊影响权值；运用模糊叠加模型，得到南沙群岛海区海底灾害地质综合评价图。与前人的研究工作进行对比的结果表明，GIS方法是进行海底灾害地质评价的一种有效方法。  相似文献   

风云卫星遥感数据高精度地理定位软件系统开发研究   总被引：1，自引：0，他引：1  

杨忠东  关敏 《遥感学报》2008,12(2):312-321

为了适应风云气象卫星遥感数据高精度地理定位要求,在研究和比较分析现有环境气象卫星遥感数据地理定位方法,尤其对其中关键的轨道计算模型进行对比研究之后,我们使用变阶变步长多步卫星轨道数值计算模型(DE/DEABM)进行气象卫星轨道计算,研制开发了新一代卫星轨道计算及遥感数据地理定位软件系统.该软件系统中卫星轨道数值积分计算模型包含了多项摄动因素计算,特别是对低轨卫星影响较大的因素,其中地球的球形引力项使用了高精度高阶EGM-96地球引力场模型,提高了非球形引力摄动计算精度,另外还考虑了太阳、月亮引力项,辐射光压摄动和大气摄动因素,使得轨道计算精度大大提高.在遥感数据定位算法开发工作中,以中国2002年5月发射的风云1号D星10通道扫描辐射计和计划2008年上半年发射的风云3号A卫星中分辨率光谱成像仪等遥感仪器为对象,比较详细地分析和研究了探测器、焦平面、主光学系统和扫描镜等遥感仪器几大关键部件的光学几何关系,提高了坐标转换系统计算精度.经过对风云1号D星多天逐日的轨道计算和定位计算试验,结果表明该软件系统24h风云1号D星轨道计算卫星矢径精度可达到几十厘米至几十米,较原来平根数分析解方法1000m左右精度有显著量级的提高.同时,风云1号D星遥感数据地理定位精度达到星下点1个像元.  相似文献   

Prediction of UT1–UTC,LOD and AAM χ<Subscript>3</Subscript> by combination of least-squares and multivariate stochastic methods     

Tomasz Niedzielski  Wiesław Kosek 《Journal of Geodesy》2008,82(2):83-92

This article presents the application of a multivariate prediction technique for predicting universal time (UT1–UTC), length of day (LOD) and the axial component of atmospheric angular momentum (AAM χ ₃). The multivariate predictions of LOD and UT1–UTC are generated by means of the combination of (1) least-squares (LS) extrapolation of models for annual, semiannual, 18.6-year, 9.3-year oscillations and for the linear trend, and (2) multivariate autoregressive (MAR) stochastic prediction of LS residuals (LS + MAR). The MAR technique enables the use of the AAM χ ₃ time-series as the explanatory variable for the computation of LOD or UT1–UTC predictions. In order to evaluate the performance of this approach, two other prediction schemes are also applied: (1) LS extrapolation, (2) combination of LS extrapolation and univariate autoregressive (AR) prediction of LS residuals (LS + AR). The multivariate predictions of AAM χ ₃ data, however, are computed as a combination of the extrapolation of the LS model for annual and semiannual oscillations and the LS + MAR. The AAM χ ₃ predictions are also compared with LS extrapolation and LS + AR prediction. It is shown that the predictions of LOD and UT1–UTC based on LS + MAR taking into account the axial component of AAM are more accurate than the predictions of LOD and UT1–UTC based on LS extrapolation or on LS + AR. In particular, the UT1–UTC predictions based on LS + MAR during El Niño/La Niña events exhibit considerably smaller prediction errors than those calculated by means of LS or LS + AR. The AAM χ ₃ time-series is predicted using LS + MAR with higher accuracy than applying LS extrapolation itself in the case of medium-term predictions (up to 100 days in the future). However, the predictions of AAM χ ₃ reveal the best accuracy for LS + AR.  相似文献